Fig. 2.

Silencing dmPFC increases perseverative errors in the 4-choice test. (A) Immunohistochemistry of c-Fos in the frontal cortex. (B) c-Fos labeling across layers (L) in dmPFC. Red rectangles: the dmPFC region analyzed. (C, D) Density of c-Fos + neurons in L2/3 (C) and L5/6 (D) dmPFC of mice with and without performing the 4-choice task. (E) Timeline of pharmacogenetic manipulation and behavioral task. (F) An example coronal section showing bilateral DREADD virus labeling in dmPFC. On average virus labeling spreads ±0.4 mm around the injection site along the anterior-posterior axis, and ±0.3 mm medial-laterally. (G) Number of trials taken to reach the performance criterion in the discrimination session. (H) Number of trials digging at each odor during the discrimination session. (I) Number of trials taken to reach the performance criterion in the reversal session. (J) Number of trials digging at each odor during the reversal session. *p < 0.05, **p < 0.01, ***p < 0.001. n = number of mice. Scale bars: 500 μm (A, F), 20 μm (B). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)