Figure 4.

MLN4924 suppressed proliferation and colony formation of endometrial cancer cells in vitro: (a) cell counting kit-8 assay showing reduced proliferation of HHUA and AN3CA cells treated with 0.3 µM MLN4924. Data presented as mean ± SD of three replicates; (b) representative images of crystal violet-stained cells, and colony forming data showing reduced colony forming ability, after 12 days in culture, of HHUA and AN3CA cells treated with 0.3 µM MLN4924 for 48 h. Data presented as mean ± SD of three independent experiments; (c) Representative flow cytometry images and data showing MLN4924-induced cell cycle arrest and apoptosis of HHUA and AN3CA cells treated with 0.3 µM MLN4924 or DMSO for 48 h. The percentages of cells in G₂/M and subG₁ phases were determined. Data presented as mean ± SD of three replicates. MLN, MLN4924; OD, optical density; PI, propidium iodide.