Figure 5.
Transgene activity in MeV-infected PDAC cells
To determine prodrug turnover in target cells, BxPC-3 cells were infected with MeV-CD-FmiRTS148a or parental MeV (MOI of 0.1). At designated time points after infection, cell lysates were collected, heat-inactivated, and incubated with 5-FC (1.5 mM) until readout. (A) Spectrophotometry. The dotted line corresponds to 1.5 mM 5-FC. (B) CD-UPRT activity over time. Absorbance data shown in (A) were normalized to MeV-treated controls and maximum activity was set to 100%. Mean values ± SD of three biological replicates are shown.