Left phrenic nerve activity (ipsilateral to C2 hemisection) during baseline and chemoreflex stimulated conditions. (A) Representative compressed traces depict raw (bottom) and integrated (top) left phrenic (i.e., ipsilateral to injury) neurograms under baseline conditions from intact rats, and rats with chronic C2 hemisection (C2Hx), exposed to 28 days of normoxia (Nx28), daily acute intermittent hypoxia (dAIH28), or chronic intermittent hypoxia simulating moderate sleep apnea (IH28-2/2). (B) Under baseline conditions, average ipsilateral/left integrated phrenic burst amplitude (volts) was reduced in all C2Hx rats vs. intact rats exposed to Nx28. In injured rat groups, no protocol-induced differences could be detected. (C) Representative traces depict raw and integrated left phrenic neurograms under chemoreflex stimulated conditions. (D) During chemoreflex stimulation, average ipsilateral/left integrated phrenic burst amplitude was reduced in C2Hx vs. intact rats. No IH protocol-specific differences were observed in injured rats. *Significantly different from intact Nx28; p < 0.05.