Table 2.

Results of the five behavioral tests at postnatal day 40 that showed no differences between mutant-control and mutant-treatment groups

Test	WT Control	WT Treatment	Mutant Control	Mutant Treatment	p-Value, All Groups	p-Value, Mutants vs. WTs	p-Value, Mutant Control vs. Mutant Treatment
Rotarod (s)	96.2 ± 18.5	144.8 ± 16.7	116 ± 17.3	117.1 ± 20.1	0.498	0.694	0.969
Grip strength
Forelimb (N)	1.1 ± 0.05	1.3 ± 0.07	0.7 ± 0.05	0.8 ± 0.08	<0.001	<0.001	0.391
Hindlimb (N)	0.7 ± 0.09	0.7 ± 0.1	0.5 ± 0.04	0.5 ± 0.05	<0.001	<0.001	0.781
T-maze (%)	65 ± 5.7	55 ± 6.5	35 ± 11.9	38 ± 8.6	0.035	0.002	0.84
Gait analysis
HL stride (cm)	6.3 ± 0.2	6.9 ± 0.07	4.4 ± 0.2	4.7 ± 0.2	<0.001	<0.001	0.375
FL stride (cm)	6.3 ± 0.2	6.8 ± 0.07	4.5 ± 0.3	4.4 ± 0.2	<0.001	<0.001	0.788
HL BOS (cm)	2.1 ± 0.07	2.2 ± 0.07	2.5 ± 0.2	2.6 ± 0.1	0.050	0.003	0.680
FL BOS (cm)	1.6 ± 0.05	1.9 ± 0.06	1.7 ± 0.2	1.5 ± 0.06	0.054	0.217	0.280
Open field
Total crosses (normalizeda)	1.3 ± 0.2	0.9 ± 0.1	1.8 ± 0.5	2.5 ± 0.2	0.003	0.001	0.068
Time in center (normalizeda)	0.6 ± 0.1	0.6 ± 0.2	1.3 ± 0.5	1.0 ± 0.2	0.128	0.02	0.953

^aNumber of crosses and time in center was divided by the mean value of the WT naive mice group and then groups were compared to reduce variability due to non-group-related factors.

BOS, base of support; FL, forelimb; HL, hindlimb.