Table 2.
Source of Variance | Cultivars (CV), df = 1 | Cadmium (Cd), df = 2 | Silicon (Si), df = 2 | CV × Cd, df = 2 | CV × Si, df = 2 | Cd × Si, df = 4 | CV × Cd × Si, df = 4 | Error, df = 36 |
---|---|---|---|---|---|---|---|---|
Root length | 62.875*** | 36.622*** | 3.922*** | 0.430*** | 0.281*** | 0.081** | 0.159*** | 0.0299 |
Shoot length | 49.832*** | 110.262*** | 2.399*** | 0.223* | 0.120ns | 0.032ns | 0.042ns | 0.068 |
Shoot fresh weight | 18.963*** | 31.062*** | 2.657*** | 0.054ns | 0.146ns | 0.113ns | 0.038ns | 0.0622 |
Shoot dry weight | 1.356*** | 1.439*** | 0.128*** | 0.005* | 0.009** | 0.004* | 0.0007ns | 0.00165 |
Root fresh weight | 3.190*** | 4.461*** | 0.481*** | 0.040** | 0.004ns | 0.018ns | 0.003ns | 0.00895 |
Root dry weight | 0.199*** | 0.355*** | 0.038*** | 0.0001ns | 0.007** | 0.0002ns | 0.00004ns | 0.00140 |
Chlorophyll a | 0.6350*** | 1.0485*** | 0.0288*** | 0.0091** | 0.00008ns | 0.00068ns | 0.00042ns | 0.00113 |
Chlorophyll b | 0.9180*** | 0.6608*** | 0.0233*** | 0.01701*** | 0.00057ns | 0.00064ns | 0.00019ns | 0.00113 |
Total chlorophyll | 3.0816*** | 3.3665*** | 0.1036*** | 0.0472*** | 0.00103ns | 0.00224ns | 0.00030ns | 0.00127 |
Total carotenoid | 0.0126*** | 0.0089*** | 2.72 × 10-4*** | 1.73 × 10-5ns | 4.31 × 10-6ns | 3.78 × 10-6ns | 3.37 × 10-6ns | 8.56 × 10-6 |
Hydrogen peroxide | 245.08*** | 95.595*** | 10.050*** | 5.845*** | 1.635*** | 0.398** | 0.111ns | 0.128 |
Malondialdehyde | 41.724*** | 21.929*** | 0.849*** | 1.273*** | 0.1599*** | 0.042** | 0.0325ns | 0.0237 |
Electrolyte leakage | 3855.18** | 2681.42*** | 197.67*** | 145.23*** | 9.62* | 1.16ns | 3.12ns | 3.28 |
TSP | 6.738*** | 3.362*** | 0.357*** | 0.085*** | 0.0103ns | 0.0032ns | 0.00146ns | 0.00490 |
Anthocyanin | 1.689*** | 2.325*** | 0.268*** | 0.014** | 0.013** | 0.006* | 0.004ns | 0.00277 |
SOD | 484.69*** | 322.47*** | 119.47*** | 2.135ns | 0.294ns | 3.999* | 0.389ns | 1.513 |
POD | 5162.5*** | 5271.11*** | 1498.09*** | 25.66ns | 15.16ns | 8.39ns | 10.45ns | 33.25 |
CAT | 121793*** | 37923*** | 9378*** | 892*** | 37ns | 81ns | 19ns | 79 |
APX | 43896*** | 25669.7*** | 6910.3*** | 404.8*** | 54.5ns | 41.3ns | 89.8* | 39.1 |
Root Fe2+ | 42629*** | 31543.5*** | 6487.1*** | 478.9*** | 1875.6*** | 34.6ns | 20.8ns | 56.9 |
Shoot Fe2+ | 20193*** | 11275.2*** | 2066.2*** | 67.2ns | 46.8ns | 41.6ns | 5.8ns | 33.7 |
Root Zn2+ | 12138** | 2681*** | 333.1*** | 232.2*** | 9.6ns | 2.5ns | 14ns | 12.6 |
Shoot Zn2+ | 1262.4*** | 497.31*** | 34.30*** | 14.64*** | 0.20ns | 0.37ns | 0.17ns | 0.88 |
Root Mg2+ | 116361*** | 25978*** | 5828*** | 770*** | 10ns | 154** | 125** | 39 |
Shoot Mg2+ | 51746*** | 25126.2*** | 3317.7*** | 109.4** | 131.6** | 49.7ns | 10.0ns | 27.6 |
Root Cd2+ | 12.033*** | 152.368*** | 11.655*** | 2.317*** | 0.034ns | 2.150*** | 0.034ns | 0.048 |
Shoot Cd2+ | 2.8921*** | 17.764*** | 1.1299*** | 0.591*** | 0.036*** | 0.1734*** | 0.0218*** | 0.0050 |
Root Si | 0.6828*** | 0.9447*** | 0.7219*** | 0.0788*** | 0.0155*** | 0.0417*** | 0.0047** | 0.00146 |
Shoot Si | 0.2790*** | 0.2440*** | 0.0221*** | 0.0085*** | 0.0032*** | 0.0004ns | 0.0005ns | 0.00051 |
Abbreviations: TSP, total soluble protein; SOD, superoxide dismutase; POD, peroxidase; CAT, catalase; APX, ascorbate peroxidase; Fe2+, iron ion; Zn2+, zinc ion; Mg2+, magnesium ion; Cd2+, cadmium; Si, silicon.
***, **, * = significant at 0.001, 0.01 and 0.05 levels, respectively.