Skip to main content
. 2021 May 19;17:1313–1322. doi: 10.3762/bjoc.17.91

Figure 2.

Figure 2

Identification of significant features associated with axenic or bacterial symbiont-associated alga Ulva mutabilis (phenotype slender). a) The structural determination was achieved by spectral matching with the analytical standards using AP-SMALDI-HRMS. b) Relative amounts of ectoine (m/z 143.0815 for [M + H]+) were determined by AP-SMALDI-HRMS measurements to compare different tissues: axenic and algae in symbiosis. One-way ANOVA with a Fisher HSD post hoc test found choline to be significant in profiles of axenic algae (F = 42, P-value < 0.0001) and ectoine in profiles of rhizoidal zones of algae in symbiosis (F = 4, P-value < 0.005) (colour code with reference to Figure 1a). c) Ectoine (m/z 143.0815 for [M + H]+, precursor ion) was identified in a single colony of the bacterial symbiont Roseovarius sp. MS2 using AP-SMALDI-HRMS/MS analysis.