Figure 4. PIM inhibition enhances virus-specific CD8 T cell response and chronic viral control.

(A and B) Representative flow cytometry plots of H2-D^b GP-33 tetramer⁺ and GP-33 peptide-stimulated CD8⁺ T cells from spleens of C57/BL6 mice infected with 2 × 10⁶ PFU LCMV clone 13 at 7 (A) and 35 (B) d.p.i. (left) and quantification of IFN-γ, CD107a⁺ and IFN-γ, TNF-α⁺ CD8 T cells stimulated with GP-33, GP-276, and NP-396 peptides (right).

(C) Serum viral titer was quantified by viral plaque assay using samples from mice treated with vehicle or PIM inhibitor at 35 d.p.i. Data are shown as mean ± SEM; *p < 0.05 by t test. Data were pooled from two independent experiments.