Figure 1. Flowchart of experiment set.

Sera of 50 patients with ACS and 50 healthy controls consisted of pooled and individual serum samples, respectively, and the samples were prepared for MS. The pooled serum samples were analyzed in information-dependent acquisition (IDA) mode to generate a library of proteins. In addition, protein identification was performed by matching the data of individual serum samples analyzed with sequential window acquisition of all theoretical fragment-ion spectra (SWATH) mode to the library. Peptides from each protein were quantified using MRM acquisition mode for validation of biomarkers identified from candidate proteins.