Table 4. List of MRM acquisition mode parameters for biomarker discovery.
No. | Uniprot accession no. | Protein name | Peptide sequence | Target ion | Q1 (m/z) | Q3 (m/z) | RT (min) | DP (V) | CE (V) |
---|---|---|---|---|---|---|---|---|---|
1 | P02763 | α-1-acid glycoprotein 1 | SDVVYTDWK | 2b3 | 556.767 | 302.135 | 4.41 | 71.7 | 28.9 |
2y5 | 556.767 | 712.330 | 4.41 | 71.7 | 28.9 | ||||
2y6 | 556.767 | 811.398 | 4.41 | 71.7 | 28.9 | ||||
2 | P01031 | Complement C5 | ATLLDIYK | 2y2 | 468.774 | 310.176 | 7.25 | 65.3 | 25.7 |
2y4 | 468.774 | 538.287 | 7.25 | 65.3 | 25.7 | ||||
2y6 | 468.774 | 764.455 | 7.25 | 65.3 | 25.7 | ||||
3 | P02750 | Leucine-rich α-2-glycoprotein | DLLLPQPDLR | 2b2 | 590.340 | 229.118 | 8.99 | 74.2 | 30.1 |
2b3 | 590.340 | 342.202 | 9.00 | 74.2 | 30.1 | ||||
2y6 | 590.340 | 725.394 | 9.00 | 74.2 | 30.1 | ||||
4 | P04004 | Vitronectin | FEDGVLDPDYPR | 2b2 | 711.830 | 277.118 | 5.90 | 83.0 | 34.5 |
2y5 | 711.830 | 647.315 | 5.90 | 83.0 | 34.5 | ||||
2y7 | 711.830 | 875.426 | 5.90 | 83.0 | 34.5 | ||||
5 | O14791 | Apolipoprotein L1 | VTEPISAESGEQVER | 2y6 | 815.900 | 717.353 | 3.07 | 90.6 | 38.2 |
2y8 | 815.900 | 933.427 | 3.08 | 90.6 | 38.2 | ||||
2y10 | 815.900 | 1091.496 | 3.08 | 90.6 | 38.2 | ||||
6 | P06396 | Gelsolin | AGALNSNDAFVLK | 2b3 | 660.351 | 200.103 | 6.13 | 79.3 | 32.6 |
2y2 | 660.351 | 260.197 | 6.12 | 79.3 | 32.6 | ||||
2y9 | 660.351 | 1007.516 | 6.13 | 79.3 | 32.6 | ||||
7 | P05452 | Tetranectin | LDTLAQEVALLK | 2b3 | 657.387 | 330.166 | 10.10 | 79.0 | 32.5 |
2y7 | 657.387 | 800.488 | 10.10 | 79.0 | 32.5 | ||||
2y8 | 657.387 | 871.525 | 10.10 | 79.0 | 32.5 |
The bold-faced target ion was used in final quantification. Abbreviations: CE, collision energy; DP, declustering potential; RT, retention time.