Fig. 7.

Upregulation of ALDOA alleviated the inhibitory effects of ARST in gliomas. The U87MG and U251 cells were transfected with the plasmids expressing empty vector (NC), ARST together with empty vector (ARST+vector), ARST together with ALDOA (ARST+ALDOA), ARST together with 1-288AA ALDOA (ARST+ 1-288ALDOA), ARST together with 78-364AA ALDOA (ARST+ 78-364ALDOA) or ARST lack of 1276-1361 nt (ARST Δ1276-1361 nt). A EdU assay was performed to detect the proliferation of the indicated cells. Scale bar = 100 μm. B Migration and invasion of the transfected cells were determined by transwell assay. Scale bar = 100 μm. C Wound-healing assay was performed to detect the migratory abilities of the indicated cells. Scale bar = 200 μm. D Confocal microscope was utilized to observe the co-staining of ALDOA and phalloidin labelled F-actin cytoskeleton in the indicated cells. Scale bar = 2 μm. E Representative micrographs of HE-stained sections of mouse brain tissues under low- (Scale bar = 1 cm) and high- (Scale bar = 200 μm) power magnifications 15 days after intracranial implantation of the U87MG cells infected with a lentiviral vector expressing NC, ARST or ARST+ALDOA. Curves show the survival rates of the xenografted mice. All results were presented as mean ± s.d. from three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001)