Table 2.

Testing performed by the hospital blood transfusion service.

Days since hospitalisation	Polyvalent DAT	IgG DAT	C3d DAT	IAS	Plasma antibody screen	Elution antibody screen
25 days	+	+	+	–	NA	Not specific in IAT
31 days	+	+	+	+	Not specific in IAT	Anti-Jka + non specific reaction
36 days	+	+	+	+	Anti-K and anti-Jka in IAT	NP
44 days	NP	NP	NP	+	Anti-K and anti-Jka in IAT	NA
47 days	+	+	+	+	Anti-K and anti-Jka in IAT and ficine	NP
55 days	+	+	–	+	Anti-K and anti-Jka in IAT	Anti-K and anti-Jka in IAT
58 days	+	+	–	+	Anti-K and anti-Jka with dosage effect in IAT and ficine	Anti-K and anti-Jka in IAT
65 days	NP	+	–	+	Anti-K in IAT	NP

DAT: Direct Antiglobulin Test/IAS: Irregular Antibody Screening/IAT: Indirect Antiglobulin Test/NA: Not Applicable/NP: Not Performed.