Figure 6. The application of PNGase F enhances the inhibitory effects of IbTX on BK (α+β1) channel. (A-B) Whole-cell current traces from deglycosylation BK channels before and after the application of IbTx (A) 100 nM and (B) 400 nM without treatment (left, CTRL) or pretreatment with PNGase F (right, PNGase F). The holding voltage was -80 mV and the currents were elicited by a pulse of +100 mV. (C) Statistical analysis of the inhibitory ratios of IbTX on BK (α+β1) channel without treatment (left, CTRL) or pretreatment with PNGase F.