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. 2021 Jun 4;9(6):e002544. doi: 10.1136/jitc-2021-002544

Figure 1.

Figure 1

1G4 transgenic mice develop T cells specific for A2Kb/NY-ESO-1157-165. (A) NY-ESO-1157–165 specific CD4+ and CD8+ T cells from various tissues of naive 9-week-old mice were identified using Tetrameric A2Kb/NY-ESO-1157–165V. Representative FACS plots are shown for peripheral blood lymphocytes (PBL), spleens, and lymph nodes (LN) in the left panels and cumulative data from three mice per group are shown as percentage of CD4+ or CD8+ T cells on the right panels. (B, C) NY-ESO-1157–165 specific CD8+ T cells from various tissues of 4-week-old, 10-week-old, and 33-week-old naïve mice were identified using Tetrameric A2Kb/NY-ESO-1157–165V. Gating strategy for B and C: SSC-A/FSC-A>FSC-A/FSC-H single cells >LiveDead/TCRbeta >TCRbeta/CD8 >Tetramer/CD44. Representative FACS plots are shown in (B) and cumulative data of 3 mice is shown in (C). *P<0.05, **p<0.001, ***p<0.0001 against 4-week-old mice in one way ANOVA, Turkey’s multiple comparisons test. ANOVA, analysis of variance; FACS, fluorescence-activated cell sorter.