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. 2021 Jun 4;9(6):e002544. doi: 10.1136/jitc-2021-002544

Figure 2.

Figure 2

Binding properties of the m1G4 TCR to, and m1G4 CD4 +and CD8+T cell responses against, NY-ESO-1 APLs. (A, B) HHD or m1G4 splenocytes were loaded with NY-ESO-1157–165 9C (blue) or 9V (Red) peptides or irrelevant control EBV280-288 or Influenza M158-66 peptides (black). CD69 upregulation on T cells was analyzed by flow cytometry for cells stained with anti-CD4 antibody (A) or anti-CD8 antibody (B). SEM error bars on duplicate or quadruplicate assays are shown. (C, D) EC50 measurement of NY-ESO-1157–165 9C and 9V peptides interaction with m1G4 CD4+ (C) or CD8+ T cells (D) after 17 hour stimulation. Mean with SEM error bars of quadruplicate assays are shown. (E) Surface plasmon resonance affinity measurements for 1G4 TCR binding to A2 complexed with NY-ESO-1157–165 9C or 9V peptides at 25°C. APLs, altered peptide ligand; TCR, T cell receptor.