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. 2021 May 24;11:664848. doi: 10.3389/fonc.2021.664848

Figure 2.

Figure 2

THZ1 downregulates the expression of mutant-type p53 rather than wild-type p53 in breast cancer cells. (A) Western blot was used to detect relative p53 protein expression in breast cancer cells after treatment with dimethyl sulfoxide (DMSO) (control) or specified concentrations of THZ1 (12.5, 25, 50, and 100 nM) for 48 h. (B) Quantification analysis of p53 protein expression after treatment with DMSO (control) or indicated concentrations of THZ1 (12.5, 25, 50, and 100 nM) for 48 h. (C) Western blot analysis of relative p53 expression levels in breast cancer cells was performed at different times(12 h, 36 h, 48 h, 60 h, and 72 h) following treatment with DMSO (control) or the same THZ1 concentration. (D) Quantification analysis of p53 protein expression after treatment with DMSO (control) or the same concentration of THZ1(100 nM) for different durations (12 h, 36 h, 48 h, 60 h, and 72 h). The mean ± standard deviation reflects the data. (n.s., non significant, P > 0.05; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n = 3).