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. 2021 May 24;11:664848. doi: 10.3389/fonc.2021.664848

Figure 3.

Figure 3

The expression of mutant-type p53 instead of wild-type p53 is downregulated after the expression of cyclin-dependent kinase 7 is blocked by LDC4297. (A) Western blot was used to detect relative p53 protein expression in breast cancer cells after treatment with dimethyl sulfoxide (DMSO) (control) or specified concentrations of LDC4297 (2.5 and 5 uM) for 48 h. (B) Quantification analysis of p53 protein expression after treatment with DMSO (control) or indicated concentrations of LDC4297 (2.5 and 5 uM) for 48 h. (C) The relative expression levels of p53 in breast cancer cells were analyzed by Western blot after treatment with DMSO (control) or the same concentration of LDC4297 (2.5 uM) for different times(12–72 h). (D) Quantification analysis of p53 protein expression after treatment with DMSO (control) or the same concentration of LDC4297 (2.5 uM) for different times (12–72 h). The mean ± standard deviation reflects the data. (n.s., non significant, P > 0.05; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n = 3).