Table 1.
Small RNA | Set of miRNA-specific probes and primers |
---|---|
miR-H2-3p | RT: 5’-GTC GTA TGC AGA GCA GGG TCC GAG GTA TTG GCA CTG CAT ACG ACA GTC GC-3’ Forward: 5’-CTG AGC CAG GGA CGA GT-3’ Probe: 5’-FAM-CGA CTG TCG TAT GCA GTG CC-BHQ-1-3’ |
miR-H3-3p | RT: 5’-GTC GTA TGC AGA GCA GGG TCC GAG GTA TTG GCA CTG CAT ACG ACG TCC CA-3’ Forward: 5’-CCA CTG GGA CTG TGC G-3’ Probe: 5’-FAM-TGG GAC GTC GTA TGC AGT GC-BHQ-1-3’ |
miR-H4-3p | RT: 5’-GTC GTA TGC AGA GCA GGG TCC GAG GTA TTC GCA CTG CAT ACG ACA CTA GC-3’ Forward: 5’-AGC CCT TGC CTG TCT AAC-3’ Probe: 5’-FAM-GCC TAG TGT CGT ATG CAG TGC-BHQ-1-3’ |
miR-H4-5p | RT: 5’-GTC GTA TGC AGA GCA GGG TCC GAG GTA TTC GCA CTG CAT ACG ACT GCT GC-3’ Forward: 5’-CGC AGG TAG AGT TTG ACA G-3’ Probe: 5’-FAM-CAA GCA GTC GTA TGC AGT GCG-BHQ-1-3’ |
miR-H5-3p | RT: 5’-GTC GTA TGC AGA GCA GGG TCC GAG GTA TTC GCA CTG CAT ACG ACC CGG AG-3’ Forward: 5’-GTC GTC AGA GAT CCA AAC C-3’ Probe: 5’-FAM-TCC GGG TCG TAT GCA GTG C-BHQ-1-3’ |
miR-H7 | RT: 5’-CTC GTA TGC AGA GCA GGG TCC GAG GTA TTC GCA CTG CAT ACG AGC CTT TG-3’ Forward: 5’-CAC AAA GGG GTC TGC AA-3’ Probe: 5’-FAM-CAA AGG GTC GTA TGC AGT GC-BHQ-1-3’ |
miR-H8 | RT: 5’-GTC GTA TGC AGA GCA GGG TCC GAG GTA TTC GCA CTG CAT ACG ACG AAC CC-3’ Forward: 5’-GAT GCG TAT ATA GGGT TCA GG-3’ Probe: 5’-FAM-GGT TCG TCG TAT GCA GTG CG-BHQ-1-3’ |
SNORD47 | RT: 5’-GTC GTA TGC AGA GCA GGG TCC GAG GTA TTC GCA CTG CAT ACG ACA ACC TC-3’ Forward: 5’-ATC ACT GTA AAA CCG TTC CA-3’ Probe: 5’-FAM-TGA TTC TGA GGT TGT CGT ATG CA-BHQ-1-3’ |
RT, reverse transcription primer