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. 2021 Feb 6;25(3):169–179. doi: 10.52547/ibj.25.3.169

Table 1.

Primers and TaqMan probes used in qRT-PCR for miRNAs

Small RNA Set of miRNA-specific probes and primers
miR-H2-3p RT: 5’-GTC GTA TGC AGA GCA GGG TCC GAG GTA TTG GCA CTG CAT ACG ACA GTC GC-3’
Forward: 5’-CTG AGC CAG GGA CGA GT-3’
Probe: 5’-FAM-CGA CTG TCG TAT GCA GTG CC-BHQ-1-3’
miR-H3-3p RT: 5’-GTC GTA TGC AGA GCA GGG TCC GAG GTA TTG GCA CTG CAT ACG ACG TCC CA-3’
Forward: 5’-CCA CTG GGA CTG TGC G-3’
Probe: 5’-FAM-TGG GAC GTC GTA TGC AGT GC-BHQ-1-3’
miR-H4-3p RT: 5’-GTC GTA TGC AGA GCA GGG TCC GAG GTA TTC GCA CTG CAT ACG ACA CTA GC-3’
Forward: 5’-AGC CCT TGC CTG TCT AAC-3’
Probe: 5’-FAM-GCC TAG TGT CGT ATG CAG TGC-BHQ-1-3’
miR-H4-5p RT: 5’-GTC GTA TGC AGA GCA GGG TCC GAG GTA TTC GCA CTG CAT ACG ACT GCT GC-3’
Forward: 5’-CGC AGG TAG AGT TTG ACA G-3’
Probe: 5’-FAM-CAA GCA GTC GTA TGC AGT GCG-BHQ-1-3’
miR-H5-3p RT: 5’-GTC GTA TGC AGA GCA GGG TCC GAG GTA TTC GCA CTG CAT ACG ACC CGG AG-3’
Forward: 5’-GTC GTC AGA GAT CCA AAC C-3’
Probe: 5’-FAM-TCC GGG TCG TAT GCA GTG C-BHQ-1-3’
miR-H7 RT: 5’-CTC GTA TGC AGA GCA GGG TCC GAG GTA TTC GCA CTG CAT ACG AGC CTT TG-3’
Forward: 5’-CAC AAA GGG GTC TGC AA-3’
Probe: 5’-FAM-CAA AGG GTC GTA TGC AGT GC-BHQ-1-3’
miR-H8 RT: 5’-GTC GTA TGC AGA GCA GGG TCC GAG GTA TTC GCA CTG CAT ACG ACG AAC CC-3’
Forward: 5’-GAT GCG TAT ATA GGGT TCA GG-3’
Probe: 5’-FAM-GGT TCG TCG TAT GCA GTG CG-BHQ-1-3’
SNORD47 RT: 5’-GTC GTA TGC AGA GCA GGG TCC GAG GTA TTC GCA CTG CAT ACG ACA ACC TC-3’
Forward: 5’-ATC ACT GTA AAA CCG TTC CA-3’
Probe: 5’-FAM-TGA TTC TGA GGT TGT CGT ATG CA-BHQ-1-3’

RT, reverse transcription primer