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. 2021 May 2;22(6):e51323. doi: 10.15252/embr.202051323

Figure EV3. Lipid transfer assays in the presence of other PTPIP51‐interacting phospholipids.

Figure EV3

Results of phospholipid transfer assays using PA‐containing liposomes supplemented with a CL mixture or PtdIns(4)P. Liposomes with different contents were tested according to the type of transfer assay.
  1. FRET‐based phospholipid transfer assay: acceptor liposomes (POPC:POPE:POPA = 50:40:10 (%)) and donor liposomes (POPC:POPE:Rhod‐PE:NBD‐PA = 50:40:2:8, POPC:POPE:Rhod‐PE:NBD‐PA/CL = 50:32:2:8:8 and 50:28:2:8:12, and POPC:POPE:Rhod‐PE:NBD‐PA/PtdIns(4)P = 50:32:2:8:8 and 50:28:2:8:12 (%)).
  2. Bead pulldown‐based phospholipid transfer assay: acceptor liposomes (100% POPC) and donor liposomes (POPC/biotinyl cap‐PE:TopFluor TMA‐PA = 97:2:1, POPC:biotinyl cap‐PE:TopFluor TMA‐PA:CL = 94:2:1:3 and 92:2:1:5 (%)).

Data information: All data are presented as the mean ± SD of technical quadruplicate experiments from one representative experiment (n = 2). The P‐value was calculated using one‐way ANOVA.