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. 2021 May 2;22(6):e51323. doi: 10.15252/embr.202051323

Figure 4. The interaction between the FFAT‐like motif of PTPIP51 and VAPB.

Figure 4

  1. A GST pulldown assay was performed to monitor the interaction between 6×His tag‐fused VAPB_ΔTM and GST‐fused PTPIP51_TPR (or GST‐fused PTPIP51_ΔTM). VAPB_ΔTM pulled down by PTPIP51_ΔTM is marked with an asterisk and highlighted with a red arrow.
  2. Schematic representation of the FFAT‐like motif of PTPIP51 and amino acid sequence alignment of the FFAT‐like motifs of human, mouse, and chicken proteins.
  3. Results of fluorescence polarization (FP) experiments to monitor the interaction between the FFAT‐like motif‐containing peptide of PTPIP51 and VAPB_ΔTM. The K d value of the interaction determined in the FP experiment was ~53 µM. Peptides with mutation of conserved phenylalanine and tyrosine residues in the FFAT motif were also tested. The data are presented as the mean ± SD of technical quadruplicate experiments.