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. 2021 Apr 14;22(6):e50684. doi: 10.15252/embr.202050684

Figure 1. USP36 promotes SUMOylation in cells.

Figure 1

  • A, B
    Overexpression of USP36 promotes SUMOylation in cells. Whole cell lysates (WCL) from H1299 (A), U2OS and HeLa (B) cells transfected with control empty vector or Flag‐USP36 were assayed by IB.
  • C–E
    Knockdown of USP36 reduces SUMOylation in cells. H1299 (C), HeLa (D), and U2OS (E) cells infected with scrambled (scr) or USP36 shRNA encoding lentiviruses were assayed by IB.
  • F
    USP36 promotes nucleolar SUMOylation. H1299 cells transfected with control or V5‐USP36 were fractionated to the cytoplasm (Cyto), nucleoplasm (Np), and nucleolus (No) fractions and assayed by IB.

Data information: SUMO2/3 conjugates are indicated in all the top panels.

Source data are available online for this figure.