Figure EV4. SUMOylation of snoRNP proteins.

• A–H
  SUMOylation of snoRNP proteins. H1299 cells transfected with the indicated snoRNP proteins together with His‐SUMO1 or His‐SUMO2 were assayed by Ni²⁺‐NTA PD and IB with anti‐Flag (A–C, E–H) or anti‐GFP (D) antibody to detect SUMOylated snoRNP proteins.
• I
  Detection of endogenous Nop58 and Nhp2 ubiquitination. H1299 cells were transfected with His‐Ub with or without Flag‐USP36 (wt or the C131A mutant) and treated with MG132 for 6 h before harvesting. The cells were then subjected to Ni²⁺‐NTA beads pulldown, followed by IB with anti‐Nop58 and anti‐Nhp2. The total His‐Ub conjugates are shown in the bottom. * indicates a non‐specific anti‐Nhp2 antibody‐reacting band.

Source data are available online for this figure.