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. 2021 Apr 14;22(6):e50684. doi: 10.15252/embr.202050684

Figure 5. USP36 interacts with snoRNPs and promotes snoRNP protein group SUMOylation.

Figure 5

  • A, B
    USP36 promotes SUMOylation of DKC1 and Nop56. H1299 cells transfected with indicated plasmids were subjected to Ni2+‐NTA PD followed by IB to detect SUMOylated Nop56 and DKC1, respectively. The protein expression was shown in the bottom of each panel.
  • C, D
    USP36 interacts with both box C/D (C) and H/ACA (D) snoRNP complexes. H1299 cells transfected with empty vector or Flag‐USP36 plasmid were assayed by IP with anti‐Flag antibody, followed by IB to detect the indicated proteins.
  • E
    USP36 interacts with snoRNP proteins in the nucleolus. The nucleoli purified from H1299 cells transfected with empty vector or Flag‐USP36 plasmid were assayed by co‐IP with anti‐Flag antibody, followed by IB to detect the indicated proteins.
  • F
    Half‐life assays. The WCL from H1299 cells treated with 50 μg/ml cycloheximide (CHX) for indicated time were assayed by IB for the indicated snoRNP proteins.
  • G
    USP36 does not change the levels of endogenous snoRNP proteins. HeLa cells infected with scrambled or the indicated USP36 shRNA lentiviruses were assayed by IB.

Source data are available online for this figure.