Skip to main content
. 2021 Apr 14;22(6):e50684. doi: 10.15252/embr.202050684

Figure 6. USP36 promotes Nop58 and Nhp2 binding to snoRNAs.

Figure 6

  • A, B
    USP36 promotes the binding of Nop58 to box C/D snoRNAs. H1299 cells transfected with Flag‐Nop58 and V5‐USP36 individually or together were assayed by RNA‐IP with anti‐Flag or control mouse IgG, followed by RT–qPCR detection of the indicated box C/D snoRNAs (A). The expression of Nop58 and USP36 proteins is shown in (B). Shown is one representative experiment of three independent experiments. Data were presented as mean ± SD, n = 3 technical replicates. *P < 0.05, compared with Nop58 alone (Student's t‐test).
  • C, D
    USP36 promotes the binding of Nhp2 to snoRNAs. H1299 cells transfected with Flag‐Nhp2 and V5‐USP36 individually or together were assayed by RNA‐IP with anti‐Flag or control mouse IgG, followed by RT–qPCR detection of the indicated box H/ACA snoRNAs (C). The expression of Nhp2 and USP36 proteins is shown in (D). Shown is one representative experiment of three independent experiments. Data were presented as mean ± SD, n = 3 technical replicates. *P < 0.05; **P < 0.01, compared with Nhp2 alone (Student's t‐test).

Source data are available online for this figure.