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. 2021 May 24;12:673912. doi: 10.3389/fimmu.2021.673912

Figure 2.

Figure 2

AAV monocytes fail to upregulate PD-L1. (A) Representative histograms and (B) summarizing scatter plot of PD-L1 surface expression of monocytes left untreated for 24 hours (n=7 each group) or after stimulation with IFNγ for 24 hours (100 IU/ml; HC n=21, AAV n=26, and RA n=7). (C) Gene expression of PD-L1 in monocytes from HC and AAV patients (n=14 each group) after stimulation with IFNγ for 24 hours measured by RT-PCR, relative to housekeeping gene β-actin. Kruskal-Wallis test with Dunn’s multiple comparisons test (B) and Mann-Whitney test (C) were applied. *P<0.05; **P<0.01. Bar graphs show mean ± SEM. AAV, ANCA-associated vasculitis; HC, healthy control donors; MFI, mean fluorescence intensity; PD-L1, Programmed death-ligand 1; RA, Rheumatoid arthritis; ANCA, Anti-neutrophil cytoplasmic antibodies; ns, statistically not significant.