Figure 6.

Inhibitory effects of HXZQ-OL on the activations of PI3K/NF-κB (A) and eicosanoid cascades (B) in IgE/Ag-mediated RBL-2H3 cells. IgE-sensitized RBL-2H3 cells were preincubated with HXZQ-OL for 30 min, followed by DNP-BSA challenge for 4 h. P-IKKα/β and p-cPLA2 were normalized to total proteins, respectively. The endogenous reference protein used for p-IκB, IκB, 5-LO, COX-2 and cytosolic NF-κB p65 was β-actin whereas for nuclear NF-κB p65 was Lamin B. The data were expressed as the mean ± SD values of three independent experiments. *p < 0.05 and **p < 0.01.