Figure 1.

Colorectal cell lines are efficiently transduced and express the transgenes

HCT116^wt, HCT116^−/- and HT29 (p53 R273H) cells were seeded in 24 wells plate (5.10⁴ cells per well). After 24 h, cell number was verified and then transduced using AdRGD-PG-eGFP (G), AdRGD-PG-p53 + AdRGD-PG-eGFP (P), AdRGD-PG-hIFNβ + AdRGD-PG-eGFP (I) AdRGD-PG-p53 + AdRGD-PG-hIFNβ (C) or no vector (O). Cells and supernatant were harvested 48 h after transduction and analyzed by the respective methodology. A. Western blot showing p53 expression. B. IFN-β measurement in cell supernatant via ELISA. These data are qualitative and included to confirm expression, thus do not conclusively indicate relative expression levels. C. Percentage of positive cells for GFP detected by flow cytometry. D. Intensity of GFP fluorescence (AU, arbitrary units). * p < .05 One Way Anova; Tukey posttest. In panels C and D, results represent the average and standard deviation of three independent experiments.