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In vitro gene-silencing efficiency in H1975 cells. Relative levels of PD-L1 mRNA (A) and protein (B) in H1975 treated with PBS or transfected with Lipo3000/NC siRNA, Lipo3000/PD-L1–siRNA, PEI/PD-L1–siRNA, HA-PEI/PD-L1–siRNA, or HA-P-PEI/siRNA and determined by qRT-PCR and western blot.
