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. 2021 Jun 6;10(1):1065–1076. doi: 10.1080/22221751.2021.1932607

Figure 3.

Figure 3.

Hypoxia prevents the infection of Vero E6 cells with pseudotyped viral particles expressing the spike protein of SARS-CoV-2. (a) Dot plots representing the infection rate of Vero E6 cells exposed to normoxia (21% O2) or hypoxia (1% O2) measured by flow cytometry (ZsGreen expression) 48 h after addition of the pseudotyped viral particles (n = 6 independent experiments, unpaired t test). Gating strategy was based on uninfected cells. Bar graphs represent infectivity relative to normoxia. (b) NCI-H460 cells are not infected by pseudotyped viral particles. Error bars represent SEM and asterisks represent p values (**** < 0001).