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. Author manuscript; available in PMC: 2021 Aug 4.
Published in final edited form as: Nat Methods. 2021 Feb 4;18(2):176–185. doi: 10.1038/s41592-020-01049-4

Extended Data Figure 3. Missing head group of the Pf80S ribosome.

Extended Data Figure 3.

A) UMAP visualization of latent space encodings of EMPIAR-10028 particles with 50 sampled points shown in black. Sampled points are ordered according to distances in latent space (Methods). Visual inspection of the 50 volumes generated at the depicted points reveals 3 volumes with the 40S in a rotated state (purple) and 6 volumes with portions of the 40S head region missing (pink).

B) Density map of the 80S ribosome with the missing head group reconstructed by cryoDRGN (pink) compared with the density maps from Figure 4C showing the canonical (blue) and 40S-rotated (purple) forms of the 80S ribosome. The density maps are generated from points 32, 4, and 1 in panel A from left to right.