Figure 3.

Blocking both ALK4 and ALK5 is required to completely inhibit SMAD activation in Braf^V600E-induced PTCs. (A and B) Expression of the Tgfβ family ligands from Affymetrix expression arrays in (A) mouse and (B) human normal thyroids and PTCs. (C) Pearson correlation coefficient between the integrated TGFβ family ligand score (TLS) and TDS using the TCGA PTC dataset (Cancer Genome Atlas Research Network 2014). (D) Western blots of thyroid tumor lysates from Braf mice treated with EW7197 and of Braf/TβR1 mice treated with follistatin.