Table 1.
Cryo-EM data collection, refinement, and validation statistics
| MDA5-TRIM65PSpry-dsRNA | RIG-I-RIPLETPSpry-dsRNA | |||
|---|---|---|---|---|
| Data collection and processing | ||||
| Magnification | 105,000 | 105,000 | ||
| Voltage (kV) | 300 | 300 | ||
| Electron exposure (e−/Å2) | 76.5/72.8 | 50 | ||
| Defocus range (μm) | −1.5 to −2.5 | −0.7 to −2.5 | ||
| Pixel size (Å) | 0.85 | 0.855 | ||
| Initial particle images (no.) | 1,230,328 | 7,072,843 | ||
| Refinement | Filament | Monomer | Filament | Monomer |
| PDB ID | 7JL2 | 7JL0 | 7JL3 | 7JL1 |
| EMDB ID | EMD-22370 | EMD-22368 | EMD-22371 | EMD-22369 |
| Final particle images (no.) | 15,306 | 49,051 | 39,718 | 74,079 |
| Map resolution (Å) | 4.3 | 4.3 | 4.2 | 3.9 |
| FSC threshold | 0.143 | 0.143 | 0.143 | 0.143 |
| Model resolution (Å) | 4.4 | 4.2 | 4.3 | 3.9 |
| FSC threshold | 0.5 | 0.5 | 0.5 | 0.5 |
| Map sharpening B factor (Å2) | −120 | −113 | −120 | −103 |
| Helical symmetry imposed | ||||
| Twist (°) | 86.941 | – | 76.1005 | – |
| Rise (Å) | 46.843 | – | 46.2706 | – |
| Model composition | ||||
| Non-hydrogen atoms | 22,122 | 7352 | 21219 | 7132 |
| Protein residues | 2,526 | 842 | 2457 | 827 |
| RNA nucleotides | 88 | 28 | 84 | 28 |
| Ligands | 12 | 4 | 12 | 4 |
| Root-mean-square deviations (RMSDs) from ideal | ||||
| Bond lengths (Å) | 0.006 | 0.008 | 0.003 | 0.010 |
| Bond angles (°) | 0.809 | 0.914 | 0.733 | 0.891 |
| Validation | ||||
| Clashscore | 18.40 | 13.08 | 11.22 | 13.12 |
| Poor rotamers (%) | 0.55 | 0.14 | 1.45 | 0.00 |
| Ramachandran plot (%) | ||||
| Favored | 94.67 | 93.29 | 97.10 | 95.65 |
| Allowed | 5.33 | 6.71 | 2.90 | 4.35 |
| Disallowed | 0.00 | 0.00 | 0.00 | 0.00 |