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. Author manuscript; available in PMC: 2022 May 24.
Published in final edited form as: Curr Biol. 2021 Mar 11;31(10):2039–2050.e7. doi: 10.1016/j.cub.2021.02.040

Figure 2. PicoGreen verification of sort fidelity.

Figure 2.

Restaining Hoechst 33342-sorted cells with PicoGreen demonstrated good separation of 1Chr- and 2Chr-sorted samples, with minimal gate leakage. The subpopulation sorted to contain >2Chr (Sort c) had an enrichment in cells with four chromosomes; however, 1Chr and 2Chr populations remained abundant. Data represents the means of four biological replicates, where each replicate included the analysis of 50,000 cells.