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. Author manuscript; available in PMC: 2021 Aug 15.
Published in final edited form as: J Immunol. 2021 Jan 8;206(4):677–685. doi: 10.4049/jimmunol.2000177

Fig. 4. Downregulation of Cbl-b increases IFN-γ secretion in IL-15-activated primary human NK cells.

Fig. 4.

(A) RNAseq was conducted using primary human NK cells transduced with either Cbl-b siRNA or Scr siRNA without or with incubation in IL-15 (10 ng/ml) for 16 h (4 donors for each group). The heatmap illustrates significantly up-regulated (red) or downregulated (green) gene expression. (B) qRT-PCR was performed to quantify the mRNA levels of IFN-γ (n=6 donors) after IL-15 (10 ng/ml) 6 h stimulation. (C) The percentage of intracellular IFN-γ+ cells in un-transduced NK cells or those transduced with Cbl-b siRNA or Scr siRNA, each activated with IL-15 (10 ng/ml) for 24 h (11.5% vs 4.338%; P=0.0008; n=5 donors). (D) Supernatants were collected after IL-15 (10 ng/ml) for 24 h and IFN-γ concentrations measured by ELISA (18115 pg/ml vs 882.5 pg/ml; P=0.0099; n=7 donors). Scr-scrambled; *P<0.05, **P<0.01, ***P<0.001 by Student’s two-tailed paired t-test or One-way ANOVA; ns, not significant. Data presented as mean ± SEM.