Table.

Details of the best predicted small interfering RNAs (siRNAs) against SARS-CoV-2

Serial number	Target region	siRNA sequences	Free energy of folding	Free energy of binding	Validation score	GC %	Tm (concentration)	Tm (Cp)
siRNA_1	23339-23357 nt	Antisense - UUAUAACACUGACACCACC Sense - GGUGGUGUCAGUGUUAUAA	1.6	−31.5	0.917	42	80.2	81.7
siRNA_2	24260-24282 nt	Antisense - AAACCUAUAAGCCAUUUGCAU Sense - GCAAAUGGCUUAUAGGUUUAA	1.9	−32.1	0.964	33	82.2	83.5
siRNA_3	24289-24311 nt	Antisense - AGAACAUUCUGUGUAACUCCA Sense - GAGUUACACAGAAUGUUCUCU	1.6	−34.1	0.781	38	80.7	81.6
siRNA_4	24295-24317 nt	Antisense - UCAUAGAGAACAUUCUGUGUA Sense - CACAGAAUGUUCUCUAUGAGA	1.9	−33.2	0.935	35	79.5	79.7

GC, guanine-cytosine; Tm, melting temperature