Supplementary Table III.

Mutations representing the old Nextstrain clade and corresponding major new Nextstrain clade nomenclatures

Nextstrain (old clades)	Mutation(s) defined for the clade	Major Nextstrain new clades (defined mutation)
A1a	ORF3a: G251V and ORF1a: L3606F	19A (-)
A3	ORF1a: L3606F and V378I
A6	nt: T514C
A7	ORF1a: A3220V
B	ORF8: L84S	19B (nt. C8782T)
B1	ORF8: L84S, nt- C18060T
B2	ORF8: L84S, nt- C29095T
B4	ORF8: L84S, N: S202N
A2	S: D614G	20A (ORF1b/nsp12b: P314L)
A2a	S: D614G, ORF1b: P314L
A2a	S: D614G, ORF1b: P314L	20B (N: R203K, G204R & ORF14: G50N)
-	-	20C (ORF1a: T265I)