TABLE 4.

Acute toxicity of the two essential oils and two chemotypes against fourth instar larvae of Ae. albopictus (ppm).

Sample code	Toxicity regression equations	4th instar larvae of Ae. albopictus
		LC20 (ppm) a	LC50 (ppm) a (95% CI)	LC80 (ppm) a
Perfume lemon essential oil	$y=118.942-120.457/\left(1+{\left(x/384.808\right)}^{3.173}\right)$ (R 2 = 0.985)	248.60	384.81 (278.464–437.534)	595.64
Perfume lemon leaves essential oil	$y=138.471-141.968/\left(1+{\left(x/395.094\right)}^{1.974}\right)$ (R 2 = 0.970)	195.73	395.09 (204.929–383.510)	797.52
(R)-(+)-limonene	$y=105.266-108.298/\left(1+{\left(x/297.369\right)}^{5.864}\right)$(R 2 = 0.975)	237.88	295.28 (255.816–328.304)	364.26
Citronellal	$y=103.437-128.585/\left(1+{\left(x/240.245\right)}^{3.219}\right)$(R 2 = 0.995)	198.53	267.08 (207.284–345.576)	382.92
Deltamethrin b	$y=118.440-153.112/\left(1+{\left(x/0.531\right)}^{1.247}\right)$ (R 2 = 0.992)	0.175	0.531 (0.055–0.148)	1.615

^a14 serial concentrations were selected to determine LC₂₀, LC₅₀ and LC₈₀ values. Mortality of each examined concentration was performed three replicates, five larvae each well was applied in this high throughput assay.

^bDeltamethrin was treated as positive control.