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. 2021 Jan;22(1):111–117. doi: 10.31557/APJCP.2021.22.1.111

Table 2.

Primers Used for Reverse-Transcription and RT-qPCR Assay of the Target miRNAs and APRIL

Target Primer sequences (5`-3`)
miR-145-5p S*: 5`- GTCGTATCGAGAGCAGGGTCCGAGGTATTCGCACTCGATACGACAGGGATT -3`
F**: 5`- GTTTTCCCAGGAATCCCT -3`
miR-185-5p S: 5`- GTCGTATCGAGAGCAGGGTCCGAGGTATTCGCACTCGATACGACTCAGGAA -3`
F: 5`- GAGAGAAAGGCAGTTCCTGA -3`
miR-103 S: 5`-GTCGTATCGAGAGCAGGGTCCGAGGTATTCGCACTCGATACGACCAAGGCA-3`
F: 5`-GCTTCTTTACAGTGCTGCC-3`
Common Reverse 5`-AGAGCAGGGTCCGAGGT-3`
APRIL F: 5`- AACAGAAGAAGCAGCACTC -3`
R: 5`- GCAGATAAACTCCAGCATCC -3`
GAPDH F:5`-GGAGTCCACTGGCGTCTTCAC-3`
R:5`-GAGGCATTGCTGATGATCTTGAGG-3`

*, Stem-loop gene-specific reverse-transcriptase primers; **, Specific forward primers for RT-qPCR amplification