Table 2.
Target | Primer sequences (5`-3`) |
---|---|
miR-145-5p | S*: 5`- GTCGTATCGAGAGCAGGGTCCGAGGTATTCGCACTCGATACGACAGGGATT -3` |
F**: 5`- GTTTTCCCAGGAATCCCT -3` | |
miR-185-5p | S: 5`- GTCGTATCGAGAGCAGGGTCCGAGGTATTCGCACTCGATACGACTCAGGAA -3` |
F: 5`- GAGAGAAAGGCAGTTCCTGA -3` | |
miR-103 | S: 5`-GTCGTATCGAGAGCAGGGTCCGAGGTATTCGCACTCGATACGACCAAGGCA-3` |
F: 5`-GCTTCTTTACAGTGCTGCC-3` | |
Common Reverse | 5`-AGAGCAGGGTCCGAGGT-3` |
APRIL | F: 5`- AACAGAAGAAGCAGCACTC -3` |
R: 5`- GCAGATAAACTCCAGCATCC -3` | |
GAPDH | F:5`-GGAGTCCACTGGCGTCTTCAC-3` |
R:5`-GAGGCATTGCTGATGATCTTGAGG-3` |
*, Stem-loop gene-specific reverse-transcriptase primers; **, Specific forward primers for RT-qPCR amplification