Figure 3.

Effects of FAK Inhibitor and Cucurbitacin B on Metastasis-Related Molecules. KKU-452 cells were incubated with (A) FAK inhibitor-14 or (B) cucurbitacin B at various concentrations for 24 h. Afterward, whole cell lysates were prepared for determination of the expression of metastasis-associated proteins using Western blot analysis. Representative figures and quantification graphs are shown. Quantitative determination of MMP-9 concentration in cell lysates was performed using ELISA kit assay. Data are mean ± SD averaged from three independent experiments. *, p < 0.05 vs respective control