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. 2021 May 21;10:e69065. doi: 10.7554/eLife.69065

Figure 4. Macaque ventromedial hypothalamic nucleus (VMH) populations revealed by single-nucleus RNA-sequencing (snRNA-seq).

(A) Schematic of experimental process for macaque snRNA-seq. (B) UMAP of 3752 VMH neuronal nuclei colored and labeled by cluster designation. (C) Expression profile of the top enriched genes for each cluster. (D) Violin plot of normalized expression for marker genes for each VMH neuronal population. (E) ESµ for the top five marker genes for each cluster determined by CELLEX. (F) Pairwise scaled expression correlation (Pearson’s r) for each macaque and mouse VMH neuronal cluster.

Figure 4.

Figure 4—figure supplement 1. Macaque single-nucleus RNA-sequencing (snRNA-seq) identifies major CNS classes.

Figure 4—figure supplement 1.

(A–C) The number of (A) cells, (B) genes, and (C) UMIs detected per sample used in this study after quality control. (D–E) UMAP of all cells, colored by (D) sample and (E) cluster. (F) Average percent of cells in each cluster across all samples. (G) Representative marker genes for each of the major CNS cell types. (H) Expression profile of top 10 enriched genes for each cluster. (I) UMAP representation of cell-type classification. (J) Quantification of cell classes per sample.
Figure 4—figure supplement 2. Identifying ventromedial hypothalamic nucleus (VMH) neurons in macaque.

Figure 4—figure supplement 2.

(A) UMAP and labeling by cluster and (B) expression of top 10 genes for each macaque neuron cluster. (C) FEZF1 and (D) NR5A1 expression across the macaque neurons. (E) Loading on the top enriched mouse Nr5a1-Cre translating ribosome affinity purification with RNA-sequencing (TRAP-seq) genes.