Skip to main content
. Author manuscript; available in PMC: 2021 Jun 8.
Published in final edited form as: Hum Gene Ther. 2001 May 20;12(8):921–932. doi: 10.1089/104303401750195881

FIG. 6.

FIG. 6.

Analysis of extratumoral spread of ZAPd-GFP by PCR amplification of GFP transgene from genomic DNA. The sensitivity of the assay was determined by amplification, using 4-fold serial dilutions of pZAPd-GFP as template in the presence of untransduced genomic DNA (top). Six hundred nanograms of DNA, extracted from tumors and various extratumoral tissues at the time of autopsy, was used in PCR analysis for the GFP transgene (middle). Shown are results with tissues taken from mice 49 days after injection of tumors with vector. Expected size of full-length amplification product is 730 base pairs. A 500-bp region of the mouse β-casein gene was amplified from the same samples as an internal control (bottom).