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. 2021 May;9(10):860. doi: 10.21037/atm-21-1908

Figure 2.

Figure 2

The effect of circHIPK3 on the proliferation, invasion, apoptosis and EMT of OSCC cells. (A) CircHIPK3 overexpression and gene knockdown models are established in OSCC cell lines Tca8113 and SCC-9, respectively. *P<0.05, **P<0.01, ***P<0.001; (B,C,D) cell proliferation is detected by the CCK-8 method and BrdU colorimetry. *P<0.05, **P<0.01, ***P<0.001. The magnification of (D) is 100×; (E) the apoptosis of Tca8113 and SCC-9 cells is detected by flow cytometry. **P<0.01; (F) Transwell assay is used to detect the invasion ability of Tca8113 and SCC-9 cells. *P<0.05, **P<0.01. Dyeing with crystal violet, the magnification is 100×; (G) EMT markers of E-cadherin, Vimentin and N-cadherin are detected by Western blot. circHIPK3, circular RNA HIPK3; OSCC, oral squamous cell carcinoma; EMT, epithelial-mesenchymal transition.