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. 2021 May;9(10):889. doi: 10.21037/atm-21-2227

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2021 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Transcriptomic heterogeneity of malignant cells in metastatic liver tissue. (A) tSNE map showing 7 cancer cell clusters. (B) Boxplot showing transcriptional diversity of cancer cells estimated by CytoTRACE (left) and differentiation trajectory of cancer cells in GBC liver metastasis (right). (C) Differences in the activity of 50 hallmark pathways scored by GSVA software. t values were calculated by a linear model. (D) GO analysis of DEGs for cluster 0 (top), cluster 3 (middle), and cluster 4 (bottom). FDR <0.05 was considered as significantly enriched. GBC, gallbladder cancer.