Figure 2.

Generation and basal phenotyping of an OIP5-AS1 KO mouse model

(A) Schematic outlining the CRISPR/Cas9 approach employed to delete the OIP5-AS1 gene (∼13.4kb) from the C57BL/6J genome. Two guide RNAs (gRNA1&2) were designed to bind at either end of the gene locus (red arrows) after which Cas9 induced removal of the entire gene by non-homologous repair mechanisms.

(B and C) (B) qPCR analysis of OIP5-AS1 gene expression from four different muscle tissues from 10-week old male wild type (WT) and OIP5-AS1 KO (KO) mice (n = 3–5/group, mean ± SEM, ∗p < 0.05 versus WT). Gastroc = Gastrocnemius (C) Body mass and organ weights of 10-week-old female WT and KO mice (n = 3–5/group, mean ± SEM). BW = body weight, LM = lean mass, FM = fat mass. For B and C, a non-parametric one-way ANOVA with multiple comparisons correction (Dunnet's) was used.