REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Anti-CFTR | UNC CFTR antibodies | ID: 596 |
Anti-CFTR MM13-4 | Merckmillipore | Cat#05-581, RRID:AB_309817 |
Anti-Calnexin | Sigma-Aldrich | Cat#C4731, RRID:AB_476845 |
Chemicals | ||
Forskolin | Sigma-Aldrich | Cat#F3917 |
VX-770 (Ivacaftor) | Selleck Chemicals | Cat#S1144 |
CFTRInh-172 | Selleck Chemicals | Cat#S7139 |
VX-770-DIAZ | (Hamilton et al., 2018) | n/a |
VX-770-BIOT | (Hamilton et al., 2018) | n/a |
Experimental models: Cell lines | ||
Human Embryonic Kidney (HEK)-293 cells | Laboratory of Dr. Daniela Rotin | n/a |
Software and algorithms | ||
ImageStudioLite | LI-COR Biosciences | https://www.licor.com/bio/image-studio-lite/download |
GraphPad Prism | GraphPad | https://www.graphpad.com/ |