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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Biomaterials. 2021 Apr 27;274:120844. doi: 10.1016/j.biomaterials.2021.120844

Figure 1. Synthesis and characterization of NP Vacc.

Figure 1.

a) Schematic drawing of PEI nanoparticle vaccine formation. PEI-PEG was conjugated with CSS-antigen via disulfide linkage (PEI conjugate) and then condensated with CpG by electrostatic complexation to form NP Vacc. b) GPC chromatograms, indicating the formation of PEI conjugate. c) Hydrodynamic size and d) surface charge of NP Vacc synthesized in different PEI conjugate:CpG weight ratios, as measured by DLS and zeta potential. e) IL-12p70 secretion and f) relative proliferation of BMDCs after incubation with PEI conjugate, CpG, or PEI NPs. g) NP Vacc composed of a PEI conjugate:CpG weight ratio of 2:1 were imaged using TEM after 2% uranyl acetate staining. Scale bar = 100 nm. Data are presented as mean ± SEM. ***p < 0.001, ****p < 0.0001, analyzed by one-way ANOVA, followed by Tukey’s HSD multiple comparison post hoc test.