Table 1:

Characterization of proteins bound to CTD.

Proteins	Binding affinity (μM)	Peptide	Sequence	Method	Ref.
Set2
Sc Set2 SRI	~6	S2,5-PCTD peptide	YSPTSPSYSPTSPSYSPTSPSC	SPR	[106]
Hs Set2 SRI	5.4±0.5	S212-P/S512-P CTD peptide	TSPSYSPTSPSYSPTSPSYSPT	SPR	[108]
Hs Set2 SRI	17.8±0.9	S212-P/S51-P CTD peptide	TSPSYSPTSPSYSPTSPS	SPR	[108]
Hs Set2 SRI	12.5±1.5	S22-P/S512-P CTD peptide	SYSPTSPSYSPTSPSYSPT	SPR	[108]
Hs Set2 SRI	5.5±0.5	S2123-P/S5123-P CTD peptide	YSPTSPSYSPTSPSYSPTSPS	SPR	[108]
Pcf11
Sc Pcf11 CID	54±6	S2-p CTD peptide	SYSPTSPSYSPTSPS	FA	[114]
Sc Pcf11 CID	180	S2-p CTD peptide	PTSPSYSPTSPS	ITC	[55]
Sc Pcf11 CID	1500±300; >1000	Unphosp. CTD peptide	YSPTSPSYSPTSPS	NMR; FA	[115]
Sc Pcf11 CID	1400±650; NBD	S21-P CTD peptide	YSPTSPSYSPTSPS	NMR; FA	[115]
Sc Pcf11 CID	240±60; NBD	S22-P CTD peptide	YSPTSPSYSPTSPS	NMR; FA	[115]
Sc Pcf11 CID	160±50; 130±35;	S212-P CTD peptide	YSPTSPSYSPTSPS	NMR; FA	[115]
Sc Pcf11 CID	630±170; 370±160/110	S212-P/ S512-P CTD peptide	YSPTSPSYSPTSPS	NMR; FA	[115]
Sc Pcf11 CID	1200±500; >1000	S512-P CTD peptide	YSPTSPSYSPTSPS	NMR; FA	[115]
Sc Pcf11 CID	127±15	S212-P CTD peptide	YSPTSPSYSPTSPS	FA	[116]
Sc Pcf11 CID	269±57	T412-P CTD peptide	YSPTSPSYSPTSPS	FA	[116]
Sp Pcf11 CID	48.1±27	S212-P CTD peptide	YSPTSPSYSPTSPS	FA	[116]
Sp Pcf11 CID	51.1±5.2	T412-P CTD peptide	YSPTSPSYSPTSPS	FA	[116]
Sc Rtt103
Sc Rtt103 CID	12±2	S2-p CTD peptide	SYSPTSPSYSPTSPS	FA	[114]
Sc Rtt103 CID	1200±300; >1000	Unphosp. CTD peptide	YSPTSPSYSPTSPS	NMR; FA	[115]
Sc Rtt103 CID	420±70; NBD	S21-P CTD peptide	YSPTSPSYSPTSPS	NMR; FA	[115]
Sc Rtt103 CID	76±16; NBD	S22-P CTD peptide	YSPTSPSYSPTSPS	NMR; FA	[115]
Sc Rtt103 CID	15±10; 2.1±0.1	S212-P CTD peptide	YSPTSPSYSPTSPS	NMR; FA	[115]
Sc Rtt103 CID	41±19; 53±10	S212-P/ S512-P CTD peptide	YSPTSPSYSPTSPS	NMR; FA	[115]
Sc Rtt103 CID	600±300; 122±2/3	S512-P CTD peptide	YSPTSPSYSPTSPS	NMR; FA	[115]
Sc Rtt103 CID	32.3±6.8	S2-P CTD peptide	YSPTSPSYSPTSPS	FA	[116]
Sc Rtt103 CID	34.8±3.2	T4-p CTD peptide	YSPTSPSYSPTSPS	FA	[116]
Sc Rtt103 CID	>1000	S5-p CTD peptide	YSPTSPSYSPTSPS	FA	[116]
Sc Rtt103 CID	6.0±0.2	S2-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[117]
Sc Rtt103 CID	43±2	S2,T4-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[117]
Sc Rtt103 CID	64±2	Unphosp. CTD peptide	PSYSPTSPSYSPTSPS	FA	[117]
Sc Rtt103 CID	NBD	Y1-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[117]
Sc Rtt103 CID	15±1	T4-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[117]
Sc Rtt103 CID	6.0±0.2	T412-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[117]
Sc Rtt103 CID	1200±300	Unphosp. CTD peptide	YSPTSPSYSPTSPS	NMR	[118]
Sc Rtt103 CID	50±10	T412-P CTD peptide	YSPTSPSYSPTSPS	NMR	[118]
Sc Rtt103 CID	9±6	S212-P CTD peptide	YSPTSPSYSPTSPS	NMR	[118]
Sc Spt6
Sc Spt6 SH2	3.6±0.15	Y1-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[114]
Sc Spt6 SH2	1.9±0.04	Y1-P-S2-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[114]
Sc Spt6 SH2	1.3±0.06	Y1-P-S5-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[114]
Sc Spt6 SH2	5.2±0.09	S5-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[114]
Sc Spt6 SH2	8.4±0.19	S2-P CTD peptide	SYSPTSPSYSPTSPS	FA	[114]
Sc Spt6 tSH2	375±69	Unphosp. CTD peptide	PSYSPTSPSYSPTSPS	FA	[119]
Sc Spt6 tSH2	38.7±2.4	(Y1-P CTD) peptide	PSYSPTSPS	FA	[119]
Sc Spt6 tSH2	45.4±1.7	Y11-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[119]
Sc Spt6 tSH2	36.8±2.6	Y12-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[119]
Sc Spt6 tSH2	5.1±0.4	Y112-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[119]
Sc Spt6 tSH2	5.7±0.4	S2,T4-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[119]
Sc Spt6 tSH2	7.2±0.3	T412-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[119]
Sc Spt6 tSH2	0.3±0.1	Y112,S512-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[119]
Sc Spt6 tSH2	0.3±0.1	Y112,S212-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[119]
Sc Spt6 tSH2	1.0±0.1	S212,S712-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[119]
Sc Spt6 tSH2	0.0152±0.0009	(Y1-P CTD) × 13	PS(YSPTSPS) × 13	MST	[119]
Nrd1
Sc Nrd1 CID	85±25	S5-p CTD peptide	PSYSPTSPSYSPTSPS	FA	[114]
Sc Nrd1 CID	126±4	S2-P CTD peptide	YSPTSPSYSPTSPS	FA	[116]
Sc Nrd1 CID	148±7	T4-p CTD peptide	YSPTSPSYSPTSPS	FA	[116]
Sc Nrd1 CID	65.8±3	S5-p CTD peptide	YSPTSPSYSPTSPS	FA	[116]
Sc Nrd1 CID	40±3	S5-P CTD peptide	YSPTSPSYSPTSPS	FA	[120]
Sc Nrd1 CID	390±30	S2-P CTD peptide	YSPTSPSYSPTSPS	FA	[120]
Sc Nrd1 CID	16±1	S2,5-P CTD peptide	YSPTSPSYSPTSPS	FA	[120]
Sc Nrd1 CID	39±3	(S5-P CTD) × 3 peptide	YSPTSPSYSPTSPSYSPTSPS	FA	[120]
Sc Nrd1 CID	700±100	Unphosp. CTD peptide	YSPTSPSYSPTSPS	FA	[121]
Sc Nrd1 CID	113±5	S512-P CTD peptide	YSPTSPSYSPTSPS	FA	[121]
Sc Nrd1 CID	140±20	S51/72-P CTD peptide	YSPTSPSYSPTSPS	FA	[121]
Sc Nrd1 CID	>1000	S712-P CTD peptide	YSPTSPSYSPTSPS	FA	[121]
Sp Seb1 CID	72.3±6.4	S2-P CTD peptide	YSPTSPSYSPTSPS	FA	[116]
Sp Seb1 CID	153.2±12.1	T4-P CTD peptide	YSPTSPSYSPTSPS	FA	[116]
Sp Seb1 CID	280.9±23.1	S5-P CTD peptide	YSPTSPSYSPTSPS	FA	[116]
FluA and FluB polymerase
Bat FluA Pol	0.9± 0.1	(S5-P)4 CTD peptide	(YSPTSPS) × 4	FA	[122]
Bat FluA Pol	6.1± 0.7	(S5-P)2 CTD peptide	(YSPTSPS) × 2	FA	[122]
Hs FluB Pol	2.9± 0.3	(S5-P)4 CTD peptide	(YSPTSPS) × 4	FA	[122]
Hs FluB Pol	4.2± 0.8	(S5-P)2 CTD peptide	(YSPTSPS) × 2	FA	[122]
Prolyl-isomerase
Sc ESS1 WW	>3000	Unphosp. CTD peptide	ASYSPTSPSYS	FA	[123]
Sc ESS1 WW	241±23	S2-P CTD peptide	ASYSPTSPSYS	FA	[123]
Sc ESS1 WW	61±4.9	S5-P CTD peptide	ASYSPTSPSYS	FA	[123]
Sc ESS1 WW	>300	Unphosp. CTD peptide	GGSGGSYSPTSPSYS	BLI	[124]
Sc ESS1 WW	2.6±0.7	S5-P CTD peptide	GGSGGSYSPTSPSYS	BLI	[124]
Sc ESS1 WW	NBD	Unphosp. CTDpeptide21	YSPTSPSYSPTSPSYSPTSPS	CD	[124]
Sc ESS1 WW	NBD	Unphosp. CTD peptide11	SPTSPSYSPTS	CD	[125]
Sc ESS1 WW	76±4	S2-P CTD9 peptide	TSPSYSPTS	CD	[125]
Sc ESS1 WW	76±4	S2-P CTD11 peptide	SPTSPSYSPTS	CD	[125]
Sc ESS1 WW	98±18	S5-P CTD9 peptide	SPTSPSYSP	CD	[125]
Sc ESS1 WW	79±13	S5-P CTD11 peptide	SPTSPSYSPTS	CD	[125]
Sc ESS1 WW	21 ± 3	S5,2-P CTD11 peptide	SPTSPSYSPTS	CD	[125]
Sc ESS1 WW	16 ±2	S2,5-P CTD14 peptide	TSPSYSPTSPSYSPS	CD	[125]
Sc ESS1 WW	17±2	S5,5,5-P CTD21 peptide	YSPTSPSYSPTSPSYSPTSPS	CD	[125]
Hs Pin1 WW	NBD	Unphosp. CTD peptide	YSPTSPS	FA	[126]
Hs Pin1 WW	61 ±6.3	S2-P CTD peptide	YSPTSPS	FA	[126]
Hs Pin1 WW	30±0.39	S5-P CTD peptide	YSPTSPS	FA	[126]
Hs Pin1 WW	10±0.8	S2-P-S5-P CTD peptide	YSPTSPS	FA	[126]
Hs Pin1 WW	NBD	Unphosp. CTD peptide	YSPTSPS	FA	[126]
Hs Pin1 WW	110± 23	S2-P CTD peptide	YSPTSPS	FA	[126]
Hs Pin1 WW	34 ± 5.9	S5-P CTD peptide	YSPTSPS	FA	[126]
Hs Pin1 WW	34 ± 6.2	S2-P-S5-P CTD peptide	YSPTSPS	FA	[126]
Hs Pin1 PPIase	NBD	Unphosp. CTD peptide	YSPTSPS	FA	[126]
Hs Pin1 PPIase	NBD	S2-P CTD peptide	YSPTSPS	FA	[126]
Hs Pin1 PPIase	> 500	S5-P CTD peptide	YSPTSPS	FA	[126]
Hs Pin1 PPIase	390 ±6.82	S2-P-S5-P CTD peptide	YSPTSPS	FA	[126]
Capping enzymes
Sp Pce1 WT	0.21 ± 0.03	(S5-P)4 CTD peptide	(YSPTSPS)×4	FA	[127]
Hs Mce 1 WT	NBD	Unphosp. CTD peptide	SPTSPSYSPTS	FA	[71]
Hs Mce1 WT	139 ± 8.5 (pH=7.0)	S2-P-S5-P CTD peptide	SPTSPSYSPTS	FA	[71]
Hs Mce1 WT	221 ± 19.5 (pH=8.0)	S2-P-S5-P CTD peptide	SPTSPSYSPTS	FA	[71]
Sc Ceg-1 WT	460.7	S5-P CTD peptide	YSPTSPSYSPTSPSYSPTSPS	FA	[128]
SCAF8
Hs SCAF8 CID	>1000	Unphosp. CTD peptide	YSPTSPSYSPTSPS	FA	[129]
Hs SCAF8 CID	68 ± 8/6	S2-P CTD peptide	YSPTSPSYSPTSPS	FA	[129]
Hs SCAF8 CID	330± 50/30	S5-P CTD peptide	YSPTSPSYSPTSPS	FA	[129]
Hs SCAF8 CID	19 ± 2	S2/5-P CTD peptide	YSPTSPSYSPTSPS	FA	[129]
Hs SCAF8 CID	90±4/3	S2/S7-P CTD peptide	YSPTSPSYSPTSPS	FA	[129]
Hs TDRD3 Tudor domain	770±30	R1810-Methylated CTD peptide	YSPSSPR(Me2a)YTPQSP	FA	[130]
PHF3
Hs PHF3 SPOC	1.6± 0.3	S2-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[131]
Hs PHF3 SPOC	0.8± 0.1	S2,7-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[131]
Hs PHF3 SPOC	4.8± 0.3	S2,5-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[131]
Hs PHF3 SPOC	20.0± 4.0	S5-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[131]
Hs PHF3 SPOC	26.0± 2.9	S7-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[131]
Hs PHF3 SPOC	5.7± 0.4	S5,7-P CTD peptide	PSYSPTSPSYSPTSPS	FA	[131]
RPRD family
Hs RPRD1A CID	339±56	Unphosp. CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD1A CID	8.4±0.7	S2-P CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD1A CID	>1000	S5-P CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD1A CID	49.8±13.7	S7-P CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD1A CID	5.2±0.5	S2,7-P CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD1A CID	>1000	S2,5-P CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD1A CID	>1000	S5,7-P CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD1A CID	48.3±5.5	S7,5-P CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD1A CID	1.0±0.1	S2-P, K7-P CTD peptide	SPKYSPTSPKYSPTSPKYS	ITC	[132]
Hs RPRD1A CID	7.42±5.37	S2-P CTD peptide	SPSYSPTSPSYSPTSPSYS	MST	[132]
Hs RPRD1B CID	114±2	Unphosp. CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD1B CID	6.8±0.2	S2-P CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD1B CID	>1000	S5-P CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD1B CID	23.6±3.2	S7-P CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD1B CID	2.6±0.2	S2,7-P CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD1B CID	>1000	S2,5-P CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD1B CID	>1000	S5,7-P CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
RPRD1B CID	30.0±4.9	S7,5-P CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
RPRD1B CID	8.3±0.5	S2-P, K7-P CTD peptide	SPKYSPTSPKYSPTSPKYS	ITC	[132]
HsRPRD2 CID	355±30	Unphosp. CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD2 CID	8.3±0.5	S2-P CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD2 CID	>1000	S5-P CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD2 CID	82.8±28.7	S7-P CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD2 CID	7.2±0.1	S2,7-P CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD2 CID	>1000	S2,5-P CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD2 CID	>1000	S5,7-P CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD2 CID	112±13	S7,5-P CTD peptide	SPSYSPTSPSYSPTSPSYS	ITC	[132]
Hs RPRD2 CID	5.2±0.1	S2-P, K7-P CTD peptide	SPKYSPTSPKYSPTSPKYS	ITC	[132]
Hs CREPT RPR	79.2±59.3	Unphosp. CTD peptide	YSPTSPSYSPTSPS	FA	[133]
Hs CREPT RPR	12.3±0.9	S212-CTD peptide	YSPTSPSYSPTSPS	FA	[133]
Hs CREPT RPR	NBD	S512-CTD peptide	YSPTSPSYSPTSPS	FA	[133]
Hs CREPT RPR	44.9±6.3	S712-CTD peptide	YSPTSPSYSPTSPS	FA	[133]
Hs p15RS RPR	40.5±8.8	Unphosp. CTD peptide	YSPTSPSYSPTSPS	FA	[133]
Hs p15RS RPR	13.7±0.8	S212-CTD peptide	YSPTSPSYSPTSPS	FA	[133]
Hs p15RS RPR	>1000	S512-CTD peptide	YSPTSPSYSPTSPS	FA	[133]
Hs p15RS RPR	62.2±3.3	S712-CTD peptide	YSPTSPSYSPTSPS	FA	[133]
FF domain
Hs TCERG1 FF4–6	102±33	S2,5-P CTD peptide	YSPTSPSYSPTSPSYSPTSPS	NMR	[134]
Hs TCERG1 FF4–6	13±5	S2,5,7-P CTD peptide	SPSYSPTSPSYSPTSPSYSPT	NMR	[134]
Hs JMJD5 NTD	1.22±0.63	Unphosp. CTD peptide	SPSYSPTSPSYSPTSPSYS	MST	[135]
Hs JMJD5 NTD	0.48±0.14	S212-P CTD peptide	SPSYSPTSPSYSPTSPSYS	MST	[135]
Hs JMJD5 NTD	1.41±0.84	S512-P CTD peptide	SPSYSPTSPSYSPTSPSYS	MST	[135]
Hs JMJD5 NTD	0.12±0.03	S21,51-S22-P CTD peptide	SPSYSPTSPSYSPTSPSYS	MST	[135]
Hs JMJD5 NTD	1.98±0.85	S2-P CTD peptide	SPTSPSYSPTSPSYSPYSK	MST	[135]
Hs JMJD5 NTD	9.92±2.63	S21, -S22,52-P CTD peptide	SPSYSPTSPSYSPTSPSYS	MST	[135]
Hs SMN Tudor domain	NBD	Unmethylated CTD peptide	YSPSSPRYTPQSP	FA	[136]
Hs SMN Tudor domain	717±66	R1810me2a Methylated CTD	YSPSSPR(me2a)YTPQSP	FA	[136]
Hs SMN Tudor domain	127±15	R1810me2s Methylated CTD	YSPSSPR(me2s)YTPQSP	FA	[136]
Hs SMN Tudor domain	175±14	R1810me2s-S Methylated CTD	SPSYSPSSPR(me2s)YTPQs	FA	[136]

Footnote: Abbreviation used in the table.

BLI: Bio-Layer Interferometry

CD: Circular Dichroism

FA: Fluorescence Anisotropy

ITC: Isothermal Titration Calorimetry

MST: Microscale Thermophoresis

NMR: Nuclear Magnetic Resonance

SPR: Surface Plasmon Resonance

Hs: Homo sapiens

Sc: Saccharomyces cerevisiae

Sp: Schizosaccharomyces pombe

NBD: No Binding Detected

NTD: N-terminal domain