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. 2021 May 12;53:101248. doi: 10.1016/j.molmet.2021.101248

Figure 1.

Figure 1

Pancreatic β-cell degranulation and dysfunction in Tg7 mice. (A) Random fed glycaemia and circulating insulin levels in 2- and 12-week-old Wt and Tg7 mice. (B) Insulin (green) immunofluorescence of pancreatic sections from wild-type (Wt) and Tg7 mice at 2w and 12w of age. Nuclei revealed by DAPI staining. Average insulin immunostaining quantification (n = 4–5). Scale bar: 75 μm. P < 0.001 12w Tg7 vs 12w Wt; p < 0.05 12w Tg7 vs 2w Tg7. (C) Flow cytometry in dissociated islets from 12w Wtβ−Tom and Tg7β−Tom mice showed the presence of β-cells with decreased granulation in mutant mice. Right: Quantification of degranulated Tom+ β-cells from Tg7 mice residing outside the Wt gate (black area considered normal β-cell granularity) expressed as fold change over Wt mice (n = 5). (D) Electron microscopy of 12w Wt and Tg7 islet preparations. Black arrowheads: mature insulin granules. Scale bar: 1 μm. Unpaired Student's t-test. Data are means ± SEM, ∗∗p < 0.01 and ∗∗∗p < 0.001.