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. 2021 May 12;53:101248. doi: 10.1016/j.molmet.2021.101248

Figure 5.

Figure 5

miR-7 overexpression induces genes involved in EMT in MIN6 cells. (A–B) Expression of miR-7 (A) and β-cell specific (B) genes in MIN6 cells infected with miR-7 (Ad-miR-7) or GFP (Ad-Ctrl) encoding adenovirus (n = 3). (C) Western blotting for β-TFs Pdx1 and Nkx6-1 and loading control γ-tubulin (n = 3). (D) mRNA levels of epithelial and mesenchymal markers in Ad-miR-7-infected MIN6 cells (n = 3) (E) Western blotting for indicated mesenchymal markers. Unpaired Student's t-test. Data are means ± SEM, ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.