FIGURE 4.

NETs promote pancreatic cancer EMT via EGFR/ERK‐dependent pathway. A, BxPC3 and MIA PaCa2 were cultured in indicated conditioned medium. Western blot analysis of the indicated proteins was performed in BxPC3 and MIA PaCa2. β‐actin was used as the loading control. B, BxPC3 and MIA PaCa2 were cultured in CM‐NETs and then treated with erlotinib (20 μM) or SCH772984 (10 nM) for 24 h. The expression levels of indicated proteins were analysed by Western blot analysis. C and D, The migratory abilities of BxPC3 and MIA PaCa2 were assessed using the wound healing assay. BxPC3 and MIA PaCa2 cells were cultured with CM‐neutrophils or CM‐NETs in the presence or absence of erlotinib (20 μM) or SCH772984 (10 nM) for 48 h. E and F, The invasive abilities of BxPC3 and MIA PaCa2 were determined using the Matrigel invasion assay. BxPC3 and MIA PaCa2 were cultured with CM‐neutrophils or CM‐NETs (added to the lower chamber) in the presence or absence of erlotinib (20 μM) or SCH772984 (10 nM) for 48 h